Biochemical characterization of proliferative and differentiated SH-SY5Y cell line as a model for Parkinson's disease

Parkinson's disease is a multifactorial neurodegenerative disease. The cellular pathology includes dopamine depletion, decrease in mitochondrial complex I enzyme activity, lysosomal glucocerebrosidase activity and glutathione levels. SH-SY5Y human neuroblastoma cell line one of the most widely used models for However, consensus on its suitability as model proliferative or differentiated state lacking. In this study, we characterized compared biochemical processes often studied PD. This phenotypes cells several differences were found. Most notably, extracellular metabolism was significantly higher SH-SY5Y. Furthermore, there greater variability levels phenotype (+/- 49%) to 16%). Finally, assay revealed significant increase phenotype. contrast, our study has found similarities between two electron transport chain tyrosine hydroxylase protein expression. results demonstrate that despite coming from same line, these possess some key their biochemistry. highlights importance careful characterization relevant pathways assess lines, such cells, modelling PD other diseases, i.e. when using but different differentiation states.